{"id":2446,"date":"2023-02-23T12:52:39","date_gmt":"2023-02-23T11:52:39","guid":{"rendered":"https:\/\/www.beachemie.com\/?post_type=maerkte&#038;p=2446"},"modified":"2026-07-17T12:04:14","modified_gmt":"2026-07-17T10:04:14","slug":"hormon-produktion","status":"publish","type":"maerkte","link":"https:\/\/beachemie.com\/en\/maerkte\/pharma\/hormon-produktion\/","title":{"rendered":"Pharma Biotech Applications"},"content":{"rendered":"<div data-elementor-type=\"wp-post\" data-elementor-id=\"2446\" class=\"elementor elementor-2446\" data-elementor-post-type=\"maerkte\">\n\t\t\t\t<div class=\"elementor-element elementor-element-768b4762 e-flex e-con-boxed e-con e-parent\" data-id=\"768b4762\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-43f45a36 e-con-full e-flex e-con e-child\" data-id=\"43f45a36\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-61bb5178 elementor-widget elementor-widget-text-editor\" data-id=\"61bb5178\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>In the upstream fermentation and downstream purification of recombinant proteins, monoclonal antibodies (mAbs), and peptide hormones (e.g. insulin), acetates are essential processing aids.<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5ad0d209 elementor-widget elementor-widget-heading\" data-id=\"5ad0d209\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Fermentation and pH control<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2ffdd4f3 elementor-widget elementor-widget-text-editor\" data-id=\"2ffdd4f3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>In microbial fermentations, ammonium acetate and potassium acetate serve a dual purpose.<\/p><p>They act as highly bioavailable, low-salinity sources of nitrogen and potassium, while the acetate anion functions as a readily metabolizable carbon source.<\/p><p>Acetate buffer systems, primarily based on sodium acetate trihydrate, are widely used to maintain precise pH control in fermenters, preventing acid-induced metabolic stress on the expression hosts.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4fccd2aa elementor-widget elementor-widget-heading\" data-id=\"4fccd2aa\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Protein purification and chromatography<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1b9f6aca elementor-widget elementor-widget-text-editor\" data-id=\"1b9f6aca\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>During the purification of therapeutic proteins, such as hormones, sodium acetate trihydrate and ammonium acetate are standard components of mobile phases.<\/p><p>In cation-exchange chromatography, an acetic acid\/sodium acetate buffer establishes the precisely controlled acidic pH required to ensure that the target protein carries a net positive charge, allowing it to bind to the column resin while impurities are washed away.<\/p><p>Furthermore, ammonium acetate is highly valued in the purification of synthetic peptides and hormones because of its volatility. During subsequent freeze-drying (lyophilization) or vacuum evaporation, the buffer sublimes completely into ammonia and acetic acid vapours, leaving behind a highly pure, salt-free API cake.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"featured_media":3306,"parent":2443,"menu_order":9,"template":"","produkte":[],"class_list":["post-2446","maerkte","type-maerkte","status-publish","has-post-thumbnail","hentry","wpbf-post"],"acf":[],"_links":{"self":[{"href":"https:\/\/beachemie.com\/en\/wp-json\/wp\/v2\/maerkte\/2446"}],"collection":[{"href":"https:\/\/beachemie.com\/en\/wp-json\/wp\/v2\/maerkte"}],"about":[{"href":"https:\/\/beachemie.com\/en\/wp-json\/wp\/v2\/types\/maerkte"}],"version-history":[{"count":0,"href":"https:\/\/beachemie.com\/en\/wp-json\/wp\/v2\/maerkte\/2446\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/beachemie.com\/en\/wp-json\/wp\/v2\/maerkte\/2443"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/beachemie.com\/en\/wp-json\/wp\/v2\/media\/3306"}],"wp:attachment":[{"href":"https:\/\/beachemie.com\/en\/wp-json\/wp\/v2\/media?parent=2446"}],"wp:term":[{"taxonomy":"produkte","embeddable":true,"href":"https:\/\/beachemie.com\/en\/wp-json\/wp\/v2\/produkte?post=2446"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}